PTX400 GEC | Alldatasheet
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Species Growth results at tip Growth factor Porphyrin Haemolysis on X V XV requirement synthesis (ALA) horse blood* H. influenzae NG NG G XV - - H. aegypticus NG NG G XV - - H. parainfluenzae NG G GV + - H. haemolyticus NG NG G XV - + H. parahaemolyticus NG G G V + + Microring® Bacterial Detectors Page 1 of 6 TECHNICAL DATA SHEET 741 Corporate Headquarters
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Warrington, PA 18976 1-800-523-2575 FAX 1-800-343-3291 Email: info@polysciences.com www.polysciences.com Europe - Germany Polysciences Europe GmbH Handelsstr. 3 D-69214 Eppelheim, Germany (49) 6221-765767 FAX (49) 6221-764620 Email: info@polysciences.de © Polysciences, Inc. Active: Jan/2007 Data Sheet #741 1 Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice any process in violation of any law or any government regulation. Polysciences offers a wide variety of products specific for microbiological and bacterial detection applications. Microring ® bacterial detectors are simple, rapid test methods which utilize specific growth factors to identify different microorganisms. Differentiation of Haemophilus species Microring® XV Cat. #24644 (50 rings/pkg) Microring® XV is a paper ring used in Haemophilus identification. It comes complete with individual printed tips containing haemin (X-factor) and nicotinamide adenine dinucleotide-NAD (V-factor) or X+V factor. This ring differentiates Haemophilus species on the basis of growth requirements for X and V factors and can be identified by their growth patterns close to the tips. V-tip also contains δ-aminolaevulinic acid (ALA) for Porphyrin test. (The porphyrin test is best performed at the start when the ring is laid on the plate prior to incubation. Using a loop take a sample of the test organism from a growing culture and spread lightly over the V-tip. Non-haemin requiring species will cause an orange-red glow to be seen from the V -tip.) Procedure 1. Spread sample of the test organism onto an agar plate of T ryptone Soy Agar or Columbia Agar (without blood). 2. Using sterile forceps, carefully place Microring ® XV in the center of the plate. Press each tip gently to ensure contact with surface. 3. Incubate plate overnight at 37° C. 4. Following incubation, growth patterns around each tip are read and used to identify the organism. X = Haemin V = NAD and δ -aminolaevulinic acid (ALA) XV = Haemin and NAD NG = No growth G = Zone of growth * Haemolysis recorded from primary culture. Microring® XV continues - pg 2
Microring® XV Interpretation Storage & Handling Store Microring® XV at 2-8°C Rings maintain a shelf life of 24 months from manufacture date. Procedure 1. Spread sample of test organism onto a plate containing anaerobic agar. 2. Using sterile forceps, carefully place Microring® AN in the center of the plate. Press each tip gently to ensure contact with surface. 3. Incubate for 24-48 hours at 35-37° C under anaerobic conditions. 4. Following incubation, growth patterns around each tip are read and used to identify the organism. Zone diameters of 15mm or greater are considerd susceptible, while those less than 15mm are regarded as resistant. © Polysciences, Inc. Active: Jan/2007 Data Sheet #741 1 Page 2 of 6 TECHNICAL DATA SHEET 741 Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice any process in violation of any law or any government regulation. Code Color Antibiotic Amount E Red Erythromycin 60ug RP Dark red Rifampicin 15ug CO White Colistin 10ug PG Pink Penicillin G 2 units K Salmon Kanamycin 1000ug VA Blue Vancomycin 5ug Microring ® AN is a filter paper ring used for the identification of Non-sporing Anaerobes. It has 6 tips which are impregnated with a range of antibiotics for the identification of species of anaerobic Gram-negative bacilli and can distinguish Gram-positive cocci from Gram-negative cocci. Gram-negative anaerobic rods are found in more than half of clinical specimens containing anaerobes. Although difficult to perform precise identifications, in practice most groups can be presumptively identified from their antibiotic susceptibility profile, which is the basis of the Microring ® AN test scheme. Microring® AN Cat. #24645 (50 rings/pkg) Species Growth factor requirements Synthesis of Haenikysis on X V porphyrins from ala horse blood H. influenzae ++ – – H. aegypticus ++ – – H. parainfluenzae –+ + – H. haemolyticus ++ – + H. parahaemolyticus –+ + + Identification Table Aesculin Growth Species E RP CO PG K VA hydrolysis in bile Indole Other details B. Fragilis S S R R RR + + + Prevotella S S V S R R – – – Black pigmented colonies with brick melaninogenicus red fluorescence. Prevotella oralis SSSSRR + – – B. ureolyticus S S S S S R – – – Oxidase positive. E = Erythromycin, RP = Rifampicin, CO = Colistin, PG = Penicillin, K = Kanamycin, V A = Vancomycin S = Sensitive, R = Resistant X = Haemin, V = NAD and δ-aminolaevulinic acid (ALA) Microring® AN continues - pg 3
B. fragilis VSRRRR Prevotella melaninogenicus S S VSRR Prevotella intermedia SSSVRR Other pigmented prevotella SSSSRR Prevotella oralis S S S S R R Non-pigmented Prevotella S S SRRR Porphyromonas SSRSRV B. ureolyticus S ISIS R F. nucleatum R SVSSR F. varium R RSSSR Gram-neg. cocci I/R S S R V R Campylobacter S R SRSR B. wadsworthia RRSRSR Desulfovibrio S RRRSR C. clostridioforme VRRRSS C. symbiosum S/I S R R S S Anaerbiospirillum RSSRSR D. pneumonsintes I I RRSR S. wadsorthensis S S S RSR L. buccalis R S SRSR C. ochracea SSRIRR © Polysciences, Inc. Active: Jan/2007 Data Sheet #741 1 Page 3 of 6 TECHNICAL DATA SHEET 741 Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice any process in violation of any law or any government regulation. Microring® AN (GIFU METHOD) Recently a new identification scheme has been developed in Japan for use with Microring ® AN. Prepare bacterial suspension as above, but adjust turbidity to between 1 and 3 MacFarland. This ensures adequate visible growth within 48hours. Using this inoculum, resistance and sensitivity are measured as follows: Aesculin Growth Species E RP CO PG K VA hydrolysis in bile Indole Other details F. nucleatum SSSSSR – – + F. varium R SRSSR – + + F. mortiferum RSRSSR + + – Gram-pos. cocci SSSSSR Gram-neg. cocci SSRSSS F. necrophorum S S S S S R – – + Lypolytic on egg yolk agar. Microring® AN Identification Continued E = Erythromycin, RP = Rifampicin, CO = Colistin, PG = Penicillin, K = Kanamycin, VA = Vancomycin S = Sensitive, R = Resistant Size of Zone (mm) <_10 11-14 >_15 Interpretation Resistant (R) Intermediate (I) Sensitive (S) E = Erythromycin RP = Rifampicin CO = Colistin PG = Penicillin K = Kanamycin VA = Vancomycin S = Sensitive R = Resistant V = Variable Storage & Handling Store Microring® AN at 2-8°C Rings maintain a shelf life of 12 months from manufacture date.
© Polysciences, Inc. Active: Jan/2007 Data Sheet #741 1 Page 4 of 6 TECHNICAL DATA SHEET 741 Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice any process in violation of any law or any government regulation. Microring® AC is a filter paper ring used for the identification of Gram-positive anaerobic cocci and some microaerophilic cocci. It has 3 individual printed tips containing various antimicrobial reagents. The resistance or sensitivity patterns can be used to help identify anaerobic cocci. In particular, P . anaerobius can be readily picked out by it’s sensitivity to SPS and P . magnus can be distinguished from P . micros by its resistance to Novobiocin. Microring® AC Cat. #24654 (50 rings/pkg) Procedure 1. Spread sample of test organism onto a plate containing anaerobic agar. 2. Using sterile forceps, carefully place Microring ® AC in the center of the plate. Press each tip gently to ensure contact with surface. 3. Incubate overnight at 37° C under anaerobic conditions. 4. Following incubation, growth patterns around each tip are read and used to identify the organism. Code Color Antibiotic Amount MZ White Metronidazole 5ug SPS Pink Sodium Polyanethol 1mg Sulphonate NO Blue Novobiocin 5ug Species Metronidazole Novobiocin SPS Indole Fatty Acids from PYG broth* Peptococcus niger S R R - B,C,iv,a Peptostreptococcus asaccharolyticus S R R + B,(A,p,l) Peptostreptococcus indolicus S R R + B,(A,p,l) Peptostreptococcus magnus SR R - A Anaerococcus prevotil S R R - B,A,(L),p Peptostreptococcus anaerobius S S S - IC,A,(iv,ib,b) Peptostreptococcus micros S S R - A(s) Peptostreptococcus productus S S R - A,l,s Peptostreptococcus tetradius S R - L,B(A,p) Atopbium parvulum S S R - L,a Staphylococcus saccharolyticus R/S R R - F/A Other microaerophilic streptococci Streptococcus intermedius R S R - L,a Gemella morbillorum R S R - L,a Streptococcus constellatus R S R - L,a Microring® AC Identification S = Suseptibility, R = Resistant, R/S = aerotolerant strains resistant (a) Occasional strains may give a zone size >12mm, but these or ganisms are easily distinguishable from Peptostreptococcus anaerobius as they are resistant to Novobicin. (b) Occasional strains may give a small zone to SPS <_ 12mm in diameter. * Gas-liquid chromotography analysis of fatty acid products in Peptone Yeast extract Glucose (PYG) broth. Capital letters indicate major metabolic products, small letters indicate minor products and parentheses indicate variable reaction. Storage & Handling Store Microring® AN at 2-8°C Rings maintain a shelf life of 12 months from manufacture date.
© Polysciences, Inc. Active: Jan/2007 Data Sheet #741 1 Page 5 of 6 TECHNICAL DATA SHEET 741 Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice any process in violation of any law or any government regulation. Microring® GV is a filter paper ring used for identification of Gardnerella vaginalis. The ring comes complete with 4 individual printed tips containing various antimicrobial reagents. Each tip of the ring contains one of the following reagents; MTZ (metronidazole), TRM (trimethoprim), STZ (sulphathiazole), and BIL, (bile salt). Each tip performs as a single identification disc because of an isolating hydrophobic barrier. On blood agar, Gardnerella is identified by sensitivity to 3 designated tips and resistance to 1 tip. Microring® GV Cat. #24655 (50 rings/pkg) Procedure 1. Spread sample of test organism onto a plate containing Columbia Agar with blood. 2. Using sterile forceps, carefully place Microring ® GV in the center of the plate. Press each tip gently to ensure contact with surface. 3. Incubate plate for 48 hours at 37° C in humid atmosphere containing 5% CO 2. 4. Following incubation, growth patterns around each tip are read and used to identify the organism. Storage & Handling Store at 4° C, avoid prolonged exposure to direct sunlight. Microring® GV should be handled with forceps. Avoid contact with skin to prevent contamination. Rings maintain a shelf life 12 of months from manufacture date. Bile 5mg Trimethoprim 5 mcg Sulphathiazole 1mg MTZ = metronidazole, TRM = trimethoprim, STZ = sulphathiazole, BIL = bile salt S = Susceptible, R = Resistant, V = Variable Species Mtz Trm Stz Bil Gardnerella Vaginalis S S R S Vaginal Lactobacilli R R V R Cat -ve Corynebacter R RVR Metronidazole 50 mcg
- Spread sample of test organism onto a plate containing anaerobic agar. 2. Using sterile forceps, carefully place Microring ® YT in the center of the plate. Press each tip gently to ensure contact with surface. 3. Incubate for 24-48 hours at 37° C under anaerobic conditions. 4. Following incubation, growth patterns around each tip are read and used to identify the organism. Storage & Handling Store Microring® YT at 2-8°C Rings maintain a shelf life of 12 months from manufacture date. Tip # Contents Color Code Concentration
1 Janus green Purple 156ug
2 Ethidium Bromide Salmon 37ug
3 Triphenol tetrazolium White 150ug
chloride (TTC)
4 Brilliant green Turquoise 200ug
5 Cycloheximide White 45ug
6 Rhodamine 6G Fuschia 60ug
© Polysciences, Inc. Active: Jan/2007 Data Sheet #741 1 Page 6 of 6 TECHNICAL DATA SHEET 741 Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice any process in violation of any law or any government regulation.
Ordering Information
Cat # Description Size
24644 Microring ® XV 1pkg (50 rings)
24645 Microring ® AN 1pkg (50 rings)
24654 Microring® AC 1pkg (50 rings)
24655 Microring ® GV 1pkg (50 rings)
24646 Microring ® YT 1pkg (50 rings)
To Order: In The U.S. Call: 1-800-523-2575 • 215-343-6484 In The U.S. Fax: 1-800-343-3291 • 215-343-0214 In Germany Call: (49) 6221-765767 In Germany Fax: (49) 6221-764620 Order online anytime at www.polysciences.com Microring® YT is a filter paper ring used for the identification of clinically important yeasts, especially Candida species. Microring ® YT uses the organism’s profile of susceptibilities to a small range of reagents as the basis of identification. Microring® YT carries 6 tips impregnated with a range of dyes and antifungal reagents. Each tip acts like an individual disc because of the isolating hydrophobic barrier. Following overnight incubation, the resistance/sensitivity results of the individual tips are used to produce a 6-figure code which is matched against a database to give the identity of the yeast. Microring® YT Cat. #24646 (50 rings/pkg)