CS-TC05 WAVELENGTH | Alldatasheet

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reported a fluorescent hypoxia biosensor based on the oxygen-dependent degradation domain of HIF-1α. tumor micro-environment that modern imaging techniques cannot provide. new vessels that help sustain the growing tumor cells. Figure 1. Tumor micro-environment, normal cells,

sensing properties of HIF-1α.

  1. An octa-arginine peptide: This controls the cell
  2. A central domain: This is from the HIF-1α
  3. The ROX fluorochrome: This long-wavelength

reporter for both in vitro and in vivo imaging. stability and degradation of the hypoxia probe. Figure 2. Biosensor design rationale. (a) Domain structures of HIF-1α. The ODD domain regulates the stability of HIF-1α

than typical imaging techniques. control parameters of the load. spectroscopy of the hypoxia sensor. Figure 4. (a) (upper panel) Chemical structures of peptide 2 and probe 1-SS. (lower panel) Hypoxia mimetics also

Case Study CS-TC05 Rev. A Page 5 © 2021 • Sales & Technical Support: (406) 587-4910 • email: sales@teamWavelength.com • web: www.teamWavelength.com KEYWORDS Hypoxia, biosensor, HIF-1a, fluorescence, fluorescent spectroscopy, peptide, photostability, oxygen-dependent degradation, ODD, angiogenesis, temperature controller, LFI-3751 PRODUCTS USED LFI-3751

REVISION HISTORY

Document Number: CS-TC05 REVISION DATE NOTES A November 2021 Initial Release REFERENCES Costoya, J. A. A Bio-inspired Hypoxia Sensor using HIF1a-Oxygen-Dependent Degradation Domain, Sci Rep 9, 7117 (2019). https://doi.org/10.1038/ s41598-019-43618-4 2. Hanahan, D. & Weinberg, R. A. Hallmarks of Cancer: the Next Generation. Cell 144, 646–674 (2011). https:// 3. Fluegen, G. et al. Phenotypic Heterogeneity of Disseminated Tumour Cells is Preset by Primary Tumour Hypoxic Microenvironments. Nat. Cell Biol. 19, 120–132 (2017). https://doi.org/10.1038/ncb3465 of HIF in tumour and stromal biology. Nat. Cell Biol. 18, 356–365 (2016). https://doi.org/10.1038/ncb3330 USEFUL LINKS

  • LFI Temperature Controller Product Page PERMISSIONS Figures 2, 3, & 4 in this case study were obtained from Reference 1. The article (Ref. 1) is distributed under terms of Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided that you give appropriate credit to the original authors and the source, provide a link to the Creative Commons license, and indicate if changes were made. No changes were made to the images or the captions. They are presented here in their original form. GLOSSARY
  • Angiogenesis - The development of new blood vessels
  • HIF-1α - The alpha subunit of the Hypoxia-Inducible Factor that helps regulate the cellular response to lack of oxygen
  • Hypoxia - Lack of oxygen
  • Neoangiogenesis - The mechanism, specifically in cancer or tumors, responsible for the formation of new blood vessels to sustain and help the tumor cells grow
  • ODD - Oxygen-Dependent Degradation
  • Photostability - Resistance to change under the influence of radiant energy and especially of light
  • Transfection - The process of artificially introducing nucleic acids (DNA or RNA) into a cell to modify the cell