D5000 HP | Alldatasheet
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Agilent D5000 ScreenTape and High Sensitivity D5000 ScreenTape Assays Accelerate DNA analysis with the Agilent
4200 TapeStation system
The Agilent 4200 TapeStation system offers automated sample processing of 1 to 96 samples for quick and reliable RNA and DNA sample quality control. The D5000 ScreenTape assay allows for the separation and analysis of DNA fragments from 100 bp to 5,000 bp, ideal for larger sized NGS libraries. Depending on the sample concentra - tion, it is possible to choose between the D5000 ScreenTape and the High Sensitivity D5000 ScreenTape application. Sample quality control analysis has never been so easy – simply load the
4200 TapeStation system with the
appropriate D5000 ScreenTape device, loading tips, and samples in tube strips or 96-well plates, and you will be review- ing results in less than 2 minutes per sample. Key Features Excellent accuracy and precision Reliably analyze size and quantity of PCR fragments, fragmented DNA, and DNA libraries. High sensitivity Qualify your precious DNA sample down to a concentration of 5 pg/μL per fragment. Easy to use Simplify your sample quality control with ready-to-use ScreenTape con - sumables and reagents. Scalable throughput Analyze any number of samples at con - stant price per sample. Fast results Obtain results in as little as one min - ute per sample independent of total sample number. Zero carryover Avoid sample carry over by running each DNA sample in a separate lane of the ScreenTape device. Minimal sample consumption Use no more than 2 μL of your pre - cious samples per run – even for high sensitivity analysis. Data Sheet Complete solution for DNA analysis
- Agilent 4200 TapeStation system (G2991AA)
- Agilent D5000 ScreenTape Assay * D5000 ScreenTape (5067-5588) * D5000 Reagents (5067-5589) * D5000 Ladder (5067-5590)
- Agilent High Sensitivity D5000 ScreenTape Assay * High Sensitivity D5000 ScreenTape (5067-5592) * High Sensitivity D5000 Reagents (5067-5593) * High Sensitivity D5000 Ladder (5067-5594)
© Agilent Technologies, Inc., 2015, 2017 Published in USA, June 1, 2017 Publication Number 5991-5211EN DNA analysis during the NGS workflow The Agilent 4200 TapeStation system and the DNA ScreenTape assays can be used at several steps of the Next gener - ation Sequencing (NGS) workflow such as shearing of genomic DNA and library amplification to ensure DNA library and sample quality. The figure shows the analysis of sheared genomic DNA for an NGS library preparation workflow with the D5000 ScreenTape assay. Agilent D5000 ScreenTape applications Learn more www.agilent.com/genomics/tapestation Buy online www.agilent.com/chem/store Find an Agilent customer center www.agilent.com/chem/contactus For Research Use Only. Not for use in diagnostic procedures. This information is subject to change without notice.
1 Signal/noise ratio > 3 for a single peak
2 Determined using D5000/High Sensitivity D5000 ladder as sample
3 Measured against 2200 TapeStation system
Sizing range 100 bp – 5,000 bp 100 bp – 5,000 bp Typical resolution 400 – 5,000 bp: 15% 400 – 5,000 bp: 15% Sensitivity 1 0.1 ng/µL 5 pg/µL Sizing precision 2 5% CV 10% CV Sizing accuracy 2 ± 10% ± 15% Quantitative precision 0.1 – 1 ng/µL: 15% CV 1 – 50 ng/µL: 10% CV 15% CV Quantitative accuracy 3 ± 20% ± 25% Quantitative range 0.1 – 50 ng/µL 10 – 1,000 pg/µL Maximum sample buffer strength 250 mM KCl 250 mM Tris-HCl 125 mM NaCl 50 mM Acetate 25 mM MgCl2 25 mM BSA 25 mM Guanidine-HCl 25 mM KCl 25 mM Tris-HCl 12.5 mM NaCl 5 mM Acetate 2.5 mM MgCl2 2.5 mM BSA 2.5 mM Guanidine-HCl Physical Specifications Agilent D5000 ScreenTape Assay Agilent High Sensitivity D5000 ScreenTape Assay Analysis time 15 samples < 25 minutes 96 samples < 135 minutes 15 samples < 20 minutes 96 samples < 120 minutes Samples per consumable 15 15 Sample volume required 1 µL 2 µL Kit stability 4 months 4 months Kit size 105 samples 105 samples