103575-100 HP | Alldatasheet
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www.agilent.com/chem For Research Use Only. Not for use in diagnostic procedures. This information is subject to change without notice. © Agilent Technologies, Inc. 2018 Printed in the USA, March 15, 2018 103575-503 Rev. A1 Part Number: 103575-100 Agilent Seahorse XF DMEM Medium pH 7.4 is for use in conjunction with Seahorse XF instruments, XF supplements, XF assay kits, cartridges and XF cell culture microplates. XF DMEM Medium pH 7.4 is formulated with 5 mM HEPES and is pre- adjusted to pH 7.4. Thus, there is no requirement to adjust the pH of the final assay medium (e.g. medium + supplements) when used under recommended conditions. The constituents are based on Dulbecco’s Modified Eagle’s Medium (DMEM). No sodium bicarbonate, glucose, glutamine/GlutaMAX or sodium pyruvate is present, allowing specific customization of the assay medium. It is recommended that this product is to be used together with Agilent Seahorse XF supplements (Glucose, Pyruvate and Glutamine, part numbers: 103577-100, 103578-100 and 103579-100, respectively). Omission of phenol red from the medium allows for the most accurate and precise measurement of absolute pH value by XF Analyzers. Other Constituents: L-Arginine•HCl, L-Cystine•2HCl, Glycine, L-Histidine•HCl•H2O, L-Isoleucine, L-Leucine, L-Lysine•HCl, L-Methionine, L-Phenylalanine, L-Serine, L-Threonine, L-Tryptophan, L-Valine, L-Tyrosine•2Na•2H 2O, Folic Acid, Riboflavin, D-Ca-Panthothenate, Choline Chloride, i-Inositol, Nicotinamide, Pyridoxine•HCl, Thiamine•HCl. Product: Agilent Seahorse XF DMEM Medium pH 7.4 (with 5 mM HEPES, without phenol red, glucose, pyruvate, and L-glutamine) Medium Specifications Osmolality 291 +/- 50 mOsm/Kg H2O Endotoxin < 0.0052 EU/mL Storage 4°C Shelf Life 12 months from the date of manufacture when stored at 4°C Medium Formulation Component Final Concentration Mg2+ (as MgSO4) 0.8 mM Ca2+ (as CaCl2) 1.8 mM NaCl 143 mM KCl 5.4 mM NaH2PO4 0.91 mM Phenol Red 0 mg/L