103336-100 HP | Alldatasheet
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Agilent Seahorse XF RPMI Medium (without Phenol Red) Data Sheet Agilent Seahorse XF RPMI Medium (without Phenol Red) (0 mM Glucose) Part number: 103336-100 Agilent Seahorse XF RPMI Medium (without Phenol Red) is for use with Agilent Seahorse Bioscience XF instruments, Agilent Seahorse XF Assay Kit cartridges, and Agilent Seahorse XF Cell Culture Microplates. XF Base Medium (without Phenol Red) has been developed to allow customization of substrates in your assay medium. The constituents are based on Roswell Park Memorial Institute Medium (RPMI). No sodium bicarbonate (tissue culture buffering agent), glucose, glutamine/GlutaMAX, or sodium pyruvate is present allowing the user to specifically customize the assay medium for their experimental design. No phenol red allows for the most accurate measurement of pH. For the most accurate measure of pH, we recommend the addition of HEPES for minimal buffering. Medium Specfications Parameter Value Osmolality 285 mOsm/Kg H2O Endotoxin < 0.0052 EU/mL Storage 4 °C Expiration 18 months when stored at 4 °C Medium Formulation Component Final concentration Mg2+ (as MgSO4) 0.4 mM Ca2+ (as Ca(NO3)2• 4H2O) 0.4 mM NaCl 102.7 mM KCl 5.4 mM NaH2PO4 5.6 mM Phenol Red 0 mg/L Other Constituents Biotin, Calcium nitrate tetrahydrate, D-Ca-Pantothenate, Choline chloride, Cyanocobalamin, Folic acid, Glycine, Glutathione, i-Inositol, L-Arginine, L-Asparagine hydrate, L-Aspartic acid, L-Cystine*2HCl, L-Glutamic acid, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine*HCl, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine*2Na*2H2O, L-Valine, Nicotinamide, para-Aminobenzoic acid, Pyridoxine*HCl, Riboflavin, and Thiamine*HCl www.agilent.com For Research Use Only. Not for use in diagnostic procedures. This information is subject to change without notice. © Agilent Technologies, Inc., 2017 Published in the USA, February 22, 2017 103336-503 Rev. B